Evaluation of recombinant Leptospira interrogans serovar canicola outer membrane proteins as diagnostic antigen.

Leptospirosis of animals and man is an important rereported in recent years are LipL32, LipL41 and OmpL1; they emerging infectious disease worldwide. In domestic animals are expressed in-vivo during infection. LipL32 and LipL41 it is an important cause of abortion, stillbirth, infertility, are important and surface-exposed leptospiral outer membrane decreased milk production and death. In dairy cattle, it is proteins expressed only in pathogenic Leptospira spp. responsible for great economic losses as a consequence of this study, recombinant LipL32 and LipL41 proteins were tested agalactia, abortion, stillbirth, birth of weak calves and reduced an antigens in ELISA against serum bovine samples. fertility. The standard serological test, such as microscopic Materials and Methods agglutination test (MAT) is time-consuming and requires technical skill. The demonstration of sero-conversion by using Growth and maintenance of bacterial strains this test requires paired acute and convalescent serum samples The leptospiral strains were maintained in semi-solid EMJH limiting it to a few reference laboratories. The other available medium. LB broth and LB agar medium were used for E. coli In